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STING loss- and gain- of function studies support a growth-restraining role in PEL. (A) BCBL1 and (B) BC3 cells were treated with 5 µM <t>H151</t> for 72 hours and subjected to CellTiter-Glo assay. Western blot of STING overexpression 48 hours after electroporation of 100 ng pcDNA3.1 (EV) or 50-100 ng STING plasmid in (C) BCBL1 and (D) BC3 cells. CellTiter-Glo assay in (E) BCBL1 and (F) BC3 cells at 72 hours after EV or STING plasmid electroporation. Data is represented as relative luminescence units. Data (A-B, E-F) are presented as mean ± SD. * indicates p<0.05; ** indicates p<0.01; *** indicates p<0.001 by Student’s t-test.
Sting Inhibitor H151, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STING loss- and gain- of function studies support a growth-restraining role in PEL. (A) BCBL1 and (B) BC3 cells were treated with 5 µM <t>H151</t> for 72 hours and subjected to CellTiter-Glo assay. Western blot of STING overexpression 48 hours after electroporation of 100 ng pcDNA3.1 (EV) or 50-100 ng STING plasmid in (C) BCBL1 and (D) BC3 cells. CellTiter-Glo assay in (E) BCBL1 and (F) BC3 cells at 72 hours after EV or STING plasmid electroporation. Data is represented as relative luminescence units. Data (A-B, E-F) are presented as mean ± SD. * indicates p<0.05; ** indicates p<0.01; *** indicates p<0.001 by Student’s t-test.
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STING loss- and gain- of function studies support a growth-restraining role in PEL. (A) BCBL1 and (B) BC3 cells were treated with 5 µM H151 for 72 hours and subjected to CellTiter-Glo assay. Western blot of STING overexpression 48 hours after electroporation of 100 ng pcDNA3.1 (EV) or 50-100 ng STING plasmid in (C) BCBL1 and (D) BC3 cells. CellTiter-Glo assay in (E) BCBL1 and (F) BC3 cells at 72 hours after EV or STING plasmid electroporation. Data is represented as relative luminescence units. Data (A-B, E-F) are presented as mean ± SD. * indicates p<0.05; ** indicates p<0.01; *** indicates p<0.001 by Student’s t-test.

Journal: Frontiers in Immunology

Article Title: STING inhibits viral lytic reactivation and cell growth in primary effusion lymphoma

doi: 10.3389/fimmu.2026.1823240

Figure Lengend Snippet: STING loss- and gain- of function studies support a growth-restraining role in PEL. (A) BCBL1 and (B) BC3 cells were treated with 5 µM H151 for 72 hours and subjected to CellTiter-Glo assay. Western blot of STING overexpression 48 hours after electroporation of 100 ng pcDNA3.1 (EV) or 50-100 ng STING plasmid in (C) BCBL1 and (D) BC3 cells. CellTiter-Glo assay in (E) BCBL1 and (F) BC3 cells at 72 hours after EV or STING plasmid electroporation. Data is represented as relative luminescence units. Data (A-B, E-F) are presented as mean ± SD. * indicates p<0.05; ** indicates p<0.01; *** indicates p<0.001 by Student’s t-test.

Article Snippet: BCBL1 and BC3 cells were treated with 5 μM STING inhibitor H151 (MedChemExpress cat# Hy-112693) ( ) prepared in DMSO for 72 hours prior to CellTiter-Glo assay.

Techniques: Glo Assay, Western Blot, Over Expression, Electroporation, Plasmid Preparation